U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

ERX1268074: Illumina HiSeq 2000 sequencing; Comparative transcriptomics of Rhizobium mesoamericanum STM3625, Cupriavidus taiwanensis LMG19424 and Burkholderia phymatum STM815 when induced by root exudates of Mimosa pudica
1 ILLUMINA (Illumina HiSeq 2000) run: 111.5M spots, 5.7G bases, 3.8Gb downloads

Design: Comparative transcriptomics of Rhizobium mesoamericanum STM3625, Cupriavidus taiwanensis LMG19424 and Burkholderia phymatum STM815 when induced by root exudates of Mimosa pudica
Submitted by: IRD (Institute of Research for Development)
Study: Comparative transcriptomics of Rhizobium mesoamericanum STM3625, Cupriavidus taiwanensis LMG19424 and Burkholderia phymatum STM815 when induced by root exudates of Mimosa pudica
PRJEB12202 • ERP013649 • All experiments • All runs
show Abstracthide Abstract
We analysed the transcriptomic response of 3 rhizobial symbionts of Mimosa pudica (Rhizobium mesoamericanum STM3625, Cupriavidus taiwanensis LMG19424 and Burkholderia phymatum STM815) when cultivated in a minimum culture medium (control condition) versus induced by root exudates of their host plant Mimosa pudica. We used RNAseq using illumina technology.
Sample: BP-Control1
SAMEA3715946 • ERS1023095 • All experiments • All runs
Organism: Paraburkholderia phymatum STM815
Library:
Name: BP-Control1
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: SINGLE
Construction protocol: Bacteria were grown in VSG medium (Vincent-Succinate-Glutamate, Vincent, 1970) supplemented with root exudates of Mimosa pudica (induced condition) or not (control condition) Total RNA were extracted at mid log pahse of growth (4,5 for BP and CT, 6h for RM) using the RiboPure kit (Ambion), and rRNA were subtracted by two steps of MicrobeExpress kit (Ambion) extracted following Libraries were built by GATC company using the Trueseq Illumina kit
Experiment attributes:
Experimental Factor: Burkholderia phymatum STM815: organism
Runs: 1 run, 111.5M spots, 5.7G bases, 3.8Gb
Run# of Spots# of BasesSizePublished
ERR1194761111,453,7025.7G3.8Gb2018-01-16

ID:
4950323

Supplemental Content

Related information

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...